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Multispectral laser-induced fluorescence imaging system for large biological samples.

作者信息

Kim Moon S, Lefcourt Alan M, Chen Yud-Ren

机构信息

Instrumentation and Sensing Laboratory, U.S. Department of Agriculture Agricultural Research Service, Building 303, Powder Mill Road, Beltsville, Maryland 20705, USA.

出版信息

Appl Opt. 2003 Jul 1;42(19):3927-34. doi: 10.1364/ao.42.003927.

DOI:10.1364/ao.42.003927
PMID:12868832
Abstract

A laser-induced fluorescence imaging system developed to capture multispectral fluorescence emission images simultaneously from a relatively large target object is described. With an expanded, 355-nm Nd:YAG laser as the excitation source, the system captures fluorescence emission images in the blue, green, red, and far-red regions of the spectrum centered at 450, 550, 678, and 730 nm, respectively, from a 30-cm-diameter target area in ambient light. Images of apples and of pork meat artificially contaminated with diluted animal feces have demonstrated the versatility of fluorescence imaging techniques for potential applications in food safety inspection. Regions of contamination, including sites that were not readily visible to the human eye, could easily be identified from the images.

摘要

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