Centeno-Lima S, do Rosário V, Parreira R, Maia A J, Freudenthal A M, Nijhof A M, Jongejan F
Centro de Malária e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Portugal.
Trop Med Int Health. 2003 Aug;8(8):760-4. doi: 10.1046/j.1365-3156.2003.01074.x.
We report the first case of human babesiosis in Portugal. A 66-year-old splenectomized man was admitted to a Lisbon hospital after 1 week of fever, abdominal pain, anorexia and nausea. A high parasitaemia (30%) of Babesia parasites was found in Giemsa-stained blood smears and, despite treatment, the patient died several weeks later of renal failure. Ethylenediaminetetraacetic acid blood samples were processed for polymerase chain reaction (PCR) and reverse line blot hybridization to confirm and characterize the Babesia infection. The amplified PCR product was cloned and subsequently sequenced. Molecular analysis showed that the infection was caused by Babesia divergens and that other blood parasites were not involved. Phylogenetic analysis showed that the 18 S ribosomal RNA gene sequence was similar to three other European isolates of B. divergens. In view of the high risk for splenectomized individuals, strict measures should be taken to avoid tick bites.
我们报告了葡萄牙首例人巴贝斯虫病病例。一名66岁的脾切除男性在出现发热、腹痛、厌食和恶心1周后入住里斯本一家医院。在吉姆萨染色的血涂片上发现了高寄生虫血症（30%）的巴贝斯虫寄生虫，尽管进行了治疗，但患者几周后死于肾衰竭。采集乙二胺四乙酸血样进行聚合酶链反应（PCR）和反向线印迹杂交，以确认和鉴定巴贝斯虫感染。扩增的PCR产物被克隆并随后测序。分子分析表明，感染是由分歧巴贝斯虫引起的，且未涉及其他血液寄生虫。系统发育分析表明，18S核糖体RNA基因序列与其他三株欧洲分歧巴贝斯虫分离株相似。鉴于脾切除个体面临的高风险，应采取严格措施避免蜱虫叮咬。
