Huber Dale L, Manginell Ronald P, Samara Michael A, Kim Byung-Il, Bunker Bruce C
Sandia National Laboratory, Post Office Box 5800, Albuquerque, NM 87185-1413, USA.
Science. 2003 Jul 18;301(5631):352-4. doi: 10.1126/science.1080759.
A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip.
一种微流控装置已被研发出来，它能够从溶液中吸附蛋白质，以可忽略不计的变性程度保持这些蛋白质，并根据指令释放它们。该装置中的活性元件是一层4纳米厚的聚合物薄膜，它可以在防污亲水状态和更疏水的蛋白质吸附状态之间进行热切换。这种活性聚合物已被集成到一个微流控热板中，该热板可以被编程在不到1秒的时间内吸附和解吸蛋白质单分子层。该装置的快速响应特性可用于蛋白质组学功能，包括在集成流体芯片上对可溶性蛋白质进行预浓缩和分离。
