[广西乙型肝炎病毒基因型分布及其临床意义]

[Distribution of hepatitis B virus genotypes and its clinical significance in Guangxi].

作者信息

Ge Xian-min, Li Dan-ya, Fang Zhong-liao, Huang Guo-yong, Jiang Shi-qiang, Pan Hai-dong, Du Yan, Wang Chao-ying, Ding Xin, Masashi Mizokami

机构信息

Guangxi Institute of Occupational Health, Nanning, Guangxi 530021, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Jun;17(2):174-9.

PMID:12870004

Abstract

OBJECTIVE

To understand the distribution of hepatitis B virus genotype in Guangxi and its clinical significance.

METHODS

Nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA in sera of asymptomatic carrier (ASC) of hepatitis B virus (HBV) and patients with different liver diseases from southern and northern Guangxi. Specimens from 161 subjects were positive for HBV DNA and HBV genotype was determined by using restriction fragment length polymorphism analysis, direct sequencing or cloning sequencing.

RESULTS

The prevalence of genotype A was 3.7% in all samples and that of genotype B, C and D was 21.7%, 72.7% and 1.2%, respectively. No other genotypes (such as genotype E, F, G, H) were found. The prevalence of genotype C showed an increasing trend in ASC, chronic hepatitis, liver cirrhosis and hepatocellular carcinoma (HCC) group; in contrast, the prevalence of genotype B showed an opposite trend, although no statistically significant difference was observed, except between ASC and HCC (P=0.05). The HBeAg positive rate was higher, and the anti-HBe positive rate was lower in patients with chronic genotype C infection than in those with genotype B (P<0.05 for both). Liver function test (ALT) abnormality was more severe in genotype C group than in genotypes A and B groups having acute or chronic infection (P<0.01 for all comparisons). The prevalence of genotype C in southern Guangxi was higher than that in northern Guangxi. In contrast, the prevalence of genotype B in southern Guangxi was lower than that in northern Guangxi.

CONCLUSIONS

The predominant HBV genotypes in Guangxi were genotypes B and C. The major genotype in southern Guangxi was genotype C; while that in northern Guangxi was genotype B, which implied that the distribution of HBV genotype C was consistent with the incidence of HCC in Guangxi. 2. Genotype C maybe associated with development of severe liver diseases including HCC. 3. Genotype A,D and B+C were mostly found in acute, hepatitis and chronic hepatitis group.

摘要

目的

了解广西乙型肝炎病毒基因型的分布及其临床意义。

方法

采用巢式聚合酶链反应（nPCR）对广西南部和北部乙型肝炎病毒（HBV）无症状携带者（ASC）及不同肝病患者血清中的HBV DNA进行扩增。对161例HBV DNA阳性标本，采用限制性片段长度多态性分析、直接测序或克隆测序法确定HBV基因型。

结果

所有样本中，A型基因型的流行率为3.7%，B型、C型和D型基因型的流行率分别为21.7%、72.7%和1.2%。未发现其他基因型（如E型、F型、G型、H型）。C型基因型在ASC、慢性肝炎、肝硬化和肝细胞癌（HCC）组中的流行率呈上升趋势；相比之下，B型基因型的流行率呈相反趋势，尽管除ASC和HCC之间外未观察到统计学显著差异（P = 0.05）。慢性C型基因型感染患者的HBeAg阳性率较高，抗-HBe阳性率低于B型基因型感染患者（两者P均<0.05）。C型基因型组的肝功能检查（ALT）异常比急性或慢性感染的A型和B型基因型组更严重（所有比较P均<0.01）。广西南部C型基因型的流行率高于广西北部。相比之下，广西南部B型基因型的流行率低于广西北部。