Lakowicz Joseph R, Malicka Joanna, Gryczynski Ignacy
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201, USA.
Photochem Photobiol. 2003 Jun;77(6):604-7. doi: 10.1562/0031-8655(2003)077<0604:iioydo>2.0.co;2.
DNA detection is usually performed using fluorescence probes. Using a DNA oligomer stained with the widely used dye 1,1'-[1,3-propanediylbis[(dimethylimino)-3,1-propanediyl]]bis[4-[(3-methyl-2(3H)-benzoxazolylidene)methyl]]-quinolinum tetraiodide (YOYO-1), we show that a substrate containing silver particles can lead to a greater than 10-fold increase in the fluorescence intensity. Proximity to silver particles also increases the photostability of YOYO-1-DNA. These results suggest that substrates or gels containing silver particles may be used for increased sensitivity in DNA detection.
DNA检测通常使用荧光探针进行。使用一种用广泛使用的染料1,1'-[1,3-丙二基双[(二甲基亚氨基)-3,1-丙二基]]双[4-[(3-甲基-2(3H)-苯并恶唑基亚基)甲基]]-喹啉四碘化物（YOYO-1）染色的DNA寡聚物，我们发现含有银颗粒的底物可使荧光强度增加10倍以上。靠近银颗粒还可提高YOYO-1-DNA的光稳定性。这些结果表明，含有银颗粒的底物或凝胶可用于提高DNA检测的灵敏度。
