Ness Jayne M, Akhtar Rizwan S, Latham Cecelia B, Roth Kevin A
Division of Pediatric Neurology, Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
J Histochem Cytochem. 2003 Aug;51(8):981-7. doi: 10.1177/002215540305100801.
Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.
在组织切片中对生物学相关蛋白质和抗原进行传统免疫荧光检测时，往往受到相对较弱信号的限制，这些信号在光照下会迅速褪色。我们开发了一种免疫组织化学方法，该方法将酪胺信号放大的灵敏度与量子点的光稳定性相结合，以克服这些限制。这种简单的方法为标准显色剂检测提供了一种灵敏且稳定的荧光免疫组织化学替代方法。
