Zhang Shuncai, Wang Wei, Ren Weiying, Dai Qian, He Boming, Zhou Kang
Department of Gastroenterology, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China.
Chin Med J (Engl). 2003 May;116(5):767-71.
To investigate the effects of lactulose on intestinal bacterial overgrowth (IBO), bacterial translocation (BT), intestinal transit and permeability in cirrhotic rats.
BT in all animals was assessed by bacterial culture of mesenteric lymph node (MLN), liver and spleen, and IBO was assessed by a jejunal bacterial count of the specific organism. Intestinal permeability was determined by the 24-hour urinary (99m)Tc-diethylenetriamine pentaacetatic acid ((99m)Tc-DTPA) excretion, and intestinal transit was determined by measuring the distribution of (51)Cr in the intestine.
BT and IBO were found in 48% and 80% of the cirrhotic rats, respectively, while not in the control rats. Cirrhotic rats with IBO had significantly higher levels of intestinal endotoxin higher rates of bacterial translocation, shorter intestinal transit time and higher intestinal permeability than those without IBO. It was also found that BT were closely associated with IBO and injury of the intestinal barrier. Compared with the placebo group, lactulose-treated rats had lower rates of BT and IBO, which were closely associated with increased intestinal transit and improved intestinal permeability by lactulose.
Our study indicate that endotoxin and bacterial translocation in cirrhotic rats may attribute to IBO and increased intestinal permeability. Lactulose that accelerates intestinal transit and improves intestinal permeability might be helpful in preventing intestinal bacterial and endotoxin translocation.
探讨乳果糖对肝硬化大鼠肠道细菌过度生长（IBO）、细菌移位（BT）、肠道转运及通透性的影响。
通过肠系膜淋巴结（MLN）、肝脏和脾脏的细菌培养评估所有动物的BT，通过特定生物体的空肠细菌计数评估IBO。通过24小时尿（99m）锝-二乙三胺五乙酸（（99m）Tc-DTPA）排泄量测定肠道通透性，通过测量（51）铬在肠道内的分布测定肠道转运。
分别在48%的肝硬化大鼠和80%的肝硬化大鼠中发现BT和IBO，而对照组大鼠未发现。与无IBO的肝硬化大鼠相比，有IBO的肝硬化大鼠肠道内毒素水平显著更高，细菌移位率更高，肠道转运时间更短，肠道通透性更高。还发现BT与IBO及肠道屏障损伤密切相关。与安慰剂组相比，乳果糖治疗的大鼠BT和IBO发生率较低，这与乳果糖促进肠道转运和改善肠道通透性密切相关。
我们的研究表明，肝硬化大鼠的内毒素和细菌移位可能归因于IBO和肠道通透性增加。加速肠道转运并改善肠道通透性的乳果糖可能有助于预防肠道细菌和内毒素移位。
