El-Enany N, Belal F, Rizk M
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt.
Farmaco. 2003 Aug;58(8):613-7. doi: 10.1016/S0014-827X(03)00081-8.
The voltammetric behavior of flubendazole was studied using direct current (DCt), differential pulse (DPP) and alternating current (ACt) polarography. The drug manifests a cathodic wave in 20% v/v formic acid solution. The wave was characterized as being irreversible, diffusion-controlled with limited adsorption properties. The diffusion current-concentration relationship was found to be rectilinear over the range 3.2-14.4 microg/ml and 0.1 to 12.8 microg/ml, using DCt and DPP modes, respectively, with minimum detectability of 0.161 microg/ml (5.14 x 10(-7) M) and 0.0.057 microg/ml (1.82 x 10(-8) M) using DCt and DPP modes, respectively. Furthermore, the proposed method was applied to the in-vitro determination of flubendazole in spiked human urine and plasma adopting the DPP technique. The percentage recoveries were 100.20+/-0.62 and 97.42+/-0.95, respectively.
采用直流极谱法（DCt）、微分脉冲极谱法（DPP）和交流极谱法（ACt）研究了氟苯达唑的伏安行为。该药物在20%（v/v）甲酸溶液中呈现一个阴极波。该波的特征为不可逆、受扩散控制且具有有限的吸附特性。分别使用DCt和DPP模式时，扩散电流与浓度的关系在3.2 - 14.4微克/毫升和0.1至12.8微克/毫升范围内呈线性，DCt和DPP模式的最低检测限分别为0.161微克/毫升（5.14×10⁻⁷摩尔/升）和0.057微克/毫升（1.82×10⁻⁸摩尔/升）。此外，采用DPP技术将所提出的方法应用于加标的人尿和血浆中氟苯达唑的体外测定。回收率分别为100.20±0.62和97.42±0.95。
