Fukuzawa T, Hamer S J, Bagnara J T
Department of Anatomy, University of Arizona, Tucson 85724.
Pigment Cell Res. 1992 Nov;5(5 Pt 1):224-9. doi: 10.1111/j.1600-0749.1992.tb00541.x.
Three types of pigment cells were isolated and cultured from larval Rana pipiens, and their attachment, maintenance, and proliferation were examined in the presence of extracellular matrix constituents (ECMs) in primary cell culture. The initial profile of pigment cell types present on day 2 of culture reflects the relative attachment of the cells to the dishes. Changes in the numbers of cells present after day 2 reflects the influence of factors present in the culture media on the maintenance, proliferation, or detachment of each type of pigment cell. Fetal bovine serum (FBS) promoted melanophore expression, but inhibited iridophore expression. FBS had no effect on xanthophores. In contrast, ventral skin conditioned medium (VCM), which contains melanization inhibiting factor, strongly stimulated iridophore expression, while it markedly inhibited melanophore expression. VCM had little effect on xanthophores. Of the ECMs tested, collagen type I had no effect on pigment cells. Fibronectin slightly inhibited melanophore expression, while it moderately stimulated iridophores and xanthophores. The stimulatory effect of fibronectin was not as strong as that of FBS or VCM. Laminin was also tested; however, it did not allow pigment cells to attach to the dishes, at least under the culture conditions utilized. The results of these experiments are discussed in terms of the general mechanisms of pigment pattern formation.
从牛蛙幼体中分离并培养了三种色素细胞，并在原代细胞培养中，研究了细胞外基质成分（ECM）存在时它们的附着、维持和增殖情况。培养第2天时存在的色素细胞类型的初始概况反映了细胞与培养皿的相对附着情况。第2天后细胞数量的变化反映了培养基中存在的因素对每种色素细胞的维持、增殖或脱离的影响。胎牛血清（FBS）促进黑素细胞的表达，但抑制虹彩细胞的表达。FBS对黄色素细胞没有影响。相反，含有黑化抑制因子的腹侧皮肤条件培养基（VCM）强烈刺激虹彩细胞的表达，同时显著抑制黑素细胞的表达。VCM对黄色素细胞影响很小。在所测试的ECM中，I型胶原对色素细胞没有影响。纤连蛋白略微抑制黑素细胞的表达，同时适度刺激虹彩细胞和黄色素细胞。纤连蛋白的刺激作用不如FBS或VCM强。还测试了层粘连蛋白；然而，至少在所采用的培养条件下，它不允许色素细胞附着到培养皿上。根据色素模式形成的一般机制对这些实验结果进行了讨论。
