Bagnara J T, Fukuzawa T
Department of Anatomy, University of Arizona, Tucson 85724.
Pigment Cell Res. 1990 Nov;3(5):243-50. doi: 10.1111/j.1600-0749.1990.tb00296.x.
That the ventral integument of adult frogs (Rana pipiens) contains factor(s) that stimulate iridophore expression (adhesion, morphologic appearance, proliferation) was demonstrated on iridophores derived from tadpoles of R. pipiens and Pachymedusa dacnicolor, and maintained in primary culture in a growth medium based upon Leibovitz's L-15. Experimental growth medium (VCM) conditioned by a one-hour exposure to pieces of ventral skin of adult R. pipiens induced iridophores to assume a broad and stellate appearance, to form confluent sheets, and to proliferate over a nine-day period. Iridophores in control medium assumed long thin profiles, detached easily, and exhibited no signs of proliferation. Unknown cells containing reflecting platelets and unusual other organelles appeared uniquely in chromatophore cultures of P. dacnicolor in VCM. The intense stimulation of iridophore expression in VCM is consistent with the known inhibitory effect of this medium on melanization and with its purported role in the determination of dorsal/ventral pigment patterns of amphibians. The results are discussed in terms of a prevailing theory about pigment cell origins and development.
成年豹蛙（Rana pipiens）腹侧皮肤中含有刺激虹膜色素细胞表达（黏附、形态外观、增殖）的因子，这一现象在源自豹蛙和达氏厚蹼蟾蝌蚪的虹膜色素细胞中得到了证实，这些细胞在基于莱博维茨L - 15的生长培养基中进行原代培养。用成年豹蛙腹侧皮肤碎片处理一小时后的实验生长培养基（VCM），能诱导虹膜色素细胞呈现宽阔的星状外观，形成融合片层，并在九天内增殖。对照培养基中的虹膜色素细胞呈细长形态，容易分离，且无增殖迹象。含有反光血小板和其他异常细胞器的未知细胞仅出现在达氏厚蹼蟾在VCM中的色素细胞培养物中。VCM对虹膜色素细胞表达的强烈刺激，与该培养基对黑色素形成的已知抑制作用及其在两栖动物背/腹色素模式决定中的假定作用相一致。根据关于色素细胞起源和发育的主流理论对结果进行了讨论。
