Santos Scott R, Gutierrez-Rodriguez Carla, Coffroth Mary Alice
Department of Biological Science, State University of New York at Buffalo, Buffalo, NY 14260-1300, USA.
Mar Biotechnol (NY). 2003 Mar-Apr;5(2):130-40. doi: 10.1007/s10126-002-0076-z.
A protocol that takes advantage of length heteroplasmy in domain V of chloroplast large subunit (cp23S)-ribosomal DNA to identify members of the symbiotic dinoflagellate genus Symbiodinium is presented. This protocol is highly specific for Symbiodinium, can provide intercladal and intracladal identification of a particular Symbiodinium isolate, and can detect multiple Symbiodinium chloroplast genotypes simultaneously in the same isolate, making his technique attractive for a variety of research questions. We used this technique to characterize variation among Symbiodinium populations associated with a range of phylogenetically diverse and geographically discrete hosts. We also examined symbiont variation within a single host, the Caribbean gorgonian Pseudopterogorgia elisabethae, from 9 sites in the Bahamas, and we report a previously undocumented level of symbiont specificity for particular members of Symbiodinium clade B in this gorgonian.
本文介绍了一种利用叶绿体大亚基（cp23S）核糖体DNA第五结构域的长度异质性来鉴定共生甲藻属（Symbiodinium）成员的方法。该方法对共生甲藻具有高度特异性，能够对特定的共生甲藻分离株进行枝间和枝内鉴定，并且可以在同一分离株中同时检测多种共生甲藻叶绿体基因型，使得这项技术对各种研究问题都具有吸引力。我们使用这项技术来描述与一系列系统发育上不同且地理上分散的宿主相关的共生甲藻种群之间的差异。我们还研究了来自巴哈马9个地点的单个宿主——加勒比柳珊瑚（Pseudopterogorgia elisabethae）内的共生体变异，并报告了该柳珊瑚中共生甲藻B枝特定成员的共生体特异性水平，这一水平此前未被记录。
