Pacaud Karine, Tritsch Denis, Burger Alain, Biellmann Jean François
Laboratoire de Chimie Organique Biologique, UMR7123, Faculté de Chimie, Université Louis Pasteur, 1 rue Blaise Pascal, FR-67008, Cedex, Strasbourg, France.
Bioorg Chem. 2003 Aug;31(4):288-305. doi: 10.1016/s0045-2068(03)00041-5.
The base exchange of nicotinamide with pyridine derivatives 1a-5a, catalyzed by pig brain NAD(+) glycohydrolase and ADP-ribosyl cyclase from Aplysia californica, generated the corresponding NAD(+) analogs 1b-5b. These analogs exhibited a high absorbance band in the visible region. The transglycosidation rate was determined by monitoring the absorbance increase. Among the tested derivatives, (E)-4-[2-(methylsulfanyl)-vinyl]-pyridine 1a was the most suitable substrate for pig brain NAD(+) glycohydrolase while 4-[1,3]-dithiolan-2-ylidenemethyl-pyridine 3a was the most efficient for ADP-ribosyl cyclase from A. californica.
由猪脑NAD(+)糖水解酶和加州海兔的ADP-核糖基环化酶催化的烟酰胺与吡啶衍生物1a - 5a的碱基交换反应，生成了相应的NAD(+)类似物1b - 5b。这些类似物在可见光区域表现出一个高吸收带。转糖苷化速率通过监测吸光度的增加来测定。在测试的衍生物中，(E)-4-[2-(甲硫基)-乙烯基]-吡啶1a是猪脑NAD(+)糖水解酶最适合的底物，而4-[1,3]-二硫杂环戊烷-2-亚甲基甲基-吡啶3a对加州海兔的ADP-核糖基环化酶最有效。
