通过聚合酶链反应诊断人细小病毒B19感染

Diagnosis of human parvovirus B19 infections by polymerase chain reaction.

作者信息

Schwarz T F, Jäger G, Holzgreve W, Roggendorf M

机构信息

Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Munich, Germany.

出版信息

Scand J Infect Dis. 1992;24(6):691-6. doi: 10.3109/00365549209062452.

DOI:10.3109/00365549209062452

PMID:1287802

Abstract

The polymerase chain reaction (PCR) was used for detecting parvovirus B19 DNA in clinical specimens. A pair of oligonucleotide primers spanning the PstI-fragment of the B19 virus genome was used for PCR, and a PCR product of 727 bp was amplified. B19 virus DNA was detected in all sera (n = 26) of individuals in the incubation period and acute phase of infection. PCR was useful for detecting viral B19 DNA in amniotic fluid and fetal blood of hydropic fetuses, confirming fetal B19 virus infection.

摘要

聚合酶链反应（PCR）用于检测临床标本中的细小病毒B19 DNA。一对跨越B19病毒基因组PstI片段的寡核苷酸引物用于PCR，扩增出一条727 bp的PCR产物。在感染潜伏期和急性期个体的所有血清（n = 26）中均检测到B19病毒DNA。PCR对于检测水肿胎儿羊水和胎儿血液中的病毒B19 DNA很有用，可确诊胎儿B19病毒感染。