Xu Liming, Kurusu Yoshikazu, Takizawa Kaori, Tanaka Junzo, Matsumoto Kojiro, Taniguchi Akiyoshi
Bionic Materials Technology Group, Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Biochem Biophys Res Commun. 2003 Aug 8;307(4):1070-4. doi: 10.1016/s0006-291x(03)01314-7.
Previous studies have shown that hST6Gal I mRNA is overexpressed in colorectal cancer tissues compared with non-malignant or benign tissue. Moreover, Form 1 (hepatic form) mRNA isoform had a marked tendency to accumulate in colon cancer [Int. J. Cancer 88 (2000) 58-65]. These findings suggest that the transcriptional regulation of Form 1 is altered during malignant transformation. We report here transcriptional regulation of the hST6Gal I gene in colon adenocarcinoma cell lines. We characterized P1 promoter region, which regulates Form 1 mRNA expression, using luciferase assays. The result indicates that the nt-156 to -1 region is important for transcriptional activity of hST6Gal I gene in colon adenocarcinoma cell lines. The nt-156 to -1 region contains HNF1 recognition element. Mutation of the HNF1 site reduced luciferase activity by about 80% compared with the wild-type construct, suggesting that HNF1 site is involved in the transcription of Form 1 mRNA in colon cancer cells.
先前的研究表明，与非恶性或良性组织相比，hST6Gal I mRNA在结直肠癌组织中过表达。此外，1型（肝脏型）mRNA亚型在结肠癌中有明显的积累趋势[《国际癌症杂志》88 (2000) 58 - 65]。这些发现表明，在恶性转化过程中，1型的转录调控发生了改变。我们在此报告结肠腺癌细胞系中hST6Gal I基因的转录调控。我们使用荧光素酶测定法对调控1型mRNA表达的P1启动子区域进行了表征。结果表明，nt - 156至 - 1区域对结肠腺癌细胞系中hST6Gal I基因的转录活性很重要。nt - 156至 - 1区域包含HNF1识别元件。与野生型构建体相比，HNF1位点的突变使荧光素酶活性降低了约80%，这表明HNF1位点参与了结肠癌细胞中1型mRNA的转录。
