Singh Rekha, Kumar Ashok, Diaz-Mitoma Francisco
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada.
Hum Immunol. 2003 Aug;64(8):780-6. doi: 10.1016/s0198-8859(03)00103-4.
B7 costimulation plays a major role in herpes simplex virus (HSV) immunity. Effects of immunoregulatory cytokines (interferon-gamma [IFN-gamma] and interleukin-10 [IL-10]) and HSV antigens on B7-1 (CD80) and B7-2 (CD86) expression on monocytes during HSV-2 infection were investigated using flow cytometry. Our results demonstrate that HSV-2 infection effects the expression of B7 isoforms (B7-1 and B7-2) on monocytes in two ways, with opposing outcomes. It abrogates the IFN-gamma upregulation of B7-1 and B7-2 and also augments expression of B7-1 and B7-2 on monocytes through an IFN-gamma independent mechanism. The clinical significance of these opposing effects may be related to the pathogenesis of recurrent HSV disease in immunocompetent hosts.
B7共刺激在单纯疱疹病毒（HSV）免疫中起主要作用。利用流式细胞术研究了免疫调节细胞因子（γ干扰素[IFN-γ]和白细胞介素-10[IL-10]）以及HSV抗原对2型单纯疱疹病毒（HSV-2）感染期间单核细胞上B7-1（CD80）和B7-2（CD86）表达的影响。我们的结果表明，HSV-2感染以两种方式影响单核细胞上B7异构体（B7-1和B7-2）的表达，结果相反。它消除了IFN-γ对B7-1和B7-2的上调作用，同时还通过一种不依赖IFN-γ的机制增强单核细胞上B7-1和B7-2的表达。这些相反作用的临床意义可能与免疫功能正常宿主中复发性HSV疾病的发病机制有关。
