Kukongviriyapan Veerapol, Prawan Auemduan, Warasiha Benjamart, Tassaneyakul Wichittra, Aiemsa-ard Jareerat
Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 40002, Thailand.
Eur J Clin Pharmacol. 2003 Aug;59(4):277-81. doi: 10.1007/s00228-003-0630-2. Epub 2003 Jul 19.
To analyze the common allele frequencies of the arylamine-N-acetyltransferase 1 (NAT1) and examine the relationship between genotype and phenotype in a Thai population.
Peripheral blood samples from 233 Thai individuals were analyzed for genotype using polymerase chain reaction with restriction fragment-length polymorphism assays and for phenotype by determination of NAT1 enzyme kinetics in leukocytes using para-aminobenozic acid as a specific substrate.
Of 466 NAT1 alleles assayed, the frequency of the NAT14 allele (wild-type) was 0.504 (95%CI 0.458-0.551) and those of the NAT110, *3 and 11 alleles were 0.438 (0.392-0.484), 0.034 (0.02-0.055) and 0.024 (0.012-0.042), respectively. Neither NAT114A nor *14B alleles were found in this studied population. The activity of NAT1 enzyme from peripheral blood leukocytes determined in 47 subjects was found to vary widely. The intrinsic clearance and Vmax values of NAT1 enzymes with genotypes NAT1 *4/*4, *10/*10 and *4/ *10 were not significantly different.
The frequency distribution of the major NAT1 alleles in the Thai population has a similar pattern to some Asian populations; however, racial differences among Asian populations need further clarification.
