通过制备电泳从鸡蛋蛋黄中纯化卵黄免疫球蛋白（IgY）。

The purification of IgY from chicken egg yolk by preparative electrophoresis.

作者信息

Gee Sarah C, Bate Irene M, Thomas Theresa M, Rylatt Dennis B

机构信息

Gradipore Ltd, 22 Rodborough Road, P.O. Box 6126, Frenchs Forest, 2086 NSW, Australia.

出版信息

Protein Expr Purif. 2003 Aug;30(2):151-5. doi: 10.1016/s1046-5928(03)00076-7.

DOI:10.1016/s1046-5928(03)00076-7

PMID:12880762

Abstract

Chicken IgY has been purified from egg yolk by preparative electrophoresis on the Gradiflow, a system which has been employed for the purification of a wide range of proteins with high recovery and biological activity. Protein purification on the Gradiflow utilises electrophoresis with selected combinations of porous membranes and buffers. The purification of IgY was achieved by initial PEG lipid precipitation, then a single step Gradiflow run by a strategy based on the relatively high pI range of IgY compared to other egg yolk proteins. The IgY yields obtained from the delipidised supernatant are consistently greater than 80% by immunoassay. The purity of the IgY fraction compared favourably with IgY prepared using three commercial products.

摘要

鸡卵黄免疫球蛋白（IgY）已通过在Gradiflow上进行制备电泳从蛋黄中纯化出来，Gradiflow是一种已用于纯化多种蛋白质且回收率高、生物活性良好的系统。在Gradiflow上进行蛋白质纯化利用了多孔膜和缓冲液的选定组合进行电泳。IgY的纯化首先通过聚乙二醇脂质沉淀实现，然后基于与其他蛋黄蛋白相比IgY相对较高的等电点范围，采用一种策略进行一步Gradiflow运行。通过免疫测定，从脱脂上清液中获得的IgY产量始终大于80%。与使用三种商业产品制备的IgY相比，IgY组分的纯度更优。