Leung D Y, Glagov S, Mathews M B
Science. 1976 Feb 6;191(4226):475-7. doi: 10.1126/science.128820.
Rabbit aortic medial cells were grown on purified elastin membranes, which were then subjected to repeated elongation and relaxation or to agitation without stretching. Cells remained attached to the membranes, and cyclic stretching resulted in a two- to fourfold increase in rates of collagen, hyaluronate, and chondroitin 6-sulfate synthesis over those in agitated or stationary preparations. Synthesis of types I and III collagen was increased to the same degree. Stretching did not increase rates of chondroitin 4-sulfate or dermatan sulfate synthesis. Differences were not attributable to differences in cell number, for DNA synthetic rates were not increased by stretching. The model system devised to demonstrate these effects provides a means for relating various modes of mechanical stimulation to cell metabolism.
兔主动脉中膜细胞在纯化的弹性蛋白膜上生长，然后对其进行反复的拉伸和松弛，或在不拉伸的情况下进行搅动。细胞仍附着在膜上，与搅动或静止状态下的样本相比，周期性拉伸使胶原蛋白、透明质酸和硫酸软骨素6-硫酸酯的合成速率提高了两到四倍。I型和III型胶原蛋白的合成增加到相同程度。拉伸并没有提高硫酸软骨素4-硫酸酯或硫酸皮肤素的合成速率。这些差异并非由细胞数量的差异所致，因为拉伸并没有提高DNA合成速率。为证明这些效应而设计的模型系统为将各种机械刺激模式与细胞代谢联系起来提供了一种方法。
