Yang Qing, Xu Jianqiang, Li Min, Lei Xuyu, An Lijia
Bioengineering Institute, Dalian University of Technology, Dalian 116012, P.R. China.
Biotechnol Lett. 2003 Apr;25(8):607-10. doi: 10.1023/a:1023067626846.
The mature gene of gloshedobin, a snake venom thrombin-like enzyme from the snake, Gloydius shedaoensis, was cloned and expressed in strain E. coli BL21 (DE3). Having been induced by IPTG, the recombinant gloshedobin was in both soluble and insoluble forms. To avoid inclusion body formation, expression was optimized at 25 degrees C. Furthermore, a 50% increase in solubilization of the target protein was obtained by adding 0.1 mM Mg2+ to the medium. The purified recombinant gloshedobin gave a 44 kDa band on SDS-PAGE gel.
