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Recovery of the proteose peptone component 3 from cheese whey in Reppal PES 100/polyethylene glycol aqueous two-phase systems.

作者信息

Rodrigues L R, Venâncio A, Teixeira J A

机构信息

Centro de Engenharia Biológica-IBQF, Universidade do Minho, Largo do Paço, 4719 Braga, Portugal.

出版信息

Biotechnol Lett. 2003 Apr;25(8):651-5. doi: 10.1023/a:1023020316445.

Abstract

Recovery of the proteose peptone component 3 from cheese whey was optimal using a 16% (w/w) Reppal PES 100--24% (w/w) PEG 600 aqueous two-phase system, at pH 7, giving a mass recovery yield of 99% and a purity of 83% for proteose peptone component 3 in the upper phase. Using the above system a partition coefficient of 30.7 and a purification factor of 6.9 were achieved.

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