在具有固定化青霉素G酰化酶的电膜反应器中进行青霉素G的连续水解。

Continuous penicillin G hydrolysis in an electro-membrane reactor with immobilized penicillin G acylase.

作者信息

Zadrazil Ales, Chmelíková Růzena, Hasal Pavel

机构信息

Institute of Chemical Technology, Prague, Department of Chemical Engineering & Center for Nonlinear Dynamics of Chemical and Biological Systems, Technická 5, 166 28 Praha 6, Czech Republic.

出版信息

Biotechnol Lett. 2003 Mar;25(6):485-90. doi: 10.1023/a:1022656129744.

DOI:10.1023/a:1022656129744

PMID:12882276

Abstract

Penicillin G (2%, w/v in phosphate buffer, pH 8) was hydrolysed in a flow-through, miniature electro-membrane reactor with the penicillin G acylase immobilized in 5% (w/v) polyacrylamide (diam. 10 mm, thickness 2.6 mm, enzyme activity 24 U ml(-1)). The conversion of penicillin G increased from 0.15 to almost 0.5 when the electric current applied to the reactor was changed from -600 to +600 A/m2 with a substrate residency of 1 h.

摘要

青霉素G（在pH 8的磷酸盐缓冲液中，2%，w/v）在一个流通式微型电膜反应器中被水解，其中青霉素G酰化酶固定在5%（w/v）的聚丙烯酰胺中（直径10毫米，厚度2.6毫米，酶活性24 U ml⁻¹）。当施加到反应器的电流从-600改变到+600 A/m²且底物停留时间为1小时时，青霉素G的转化率从0.15增加到几乎0.5。