Wang Li, Wang Zhilong, Xu Jian-He, Bao Da, Qi Hanshi
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Bioprocess Biosyst Eng. 2006 Aug;29(3):157-62. doi: 10.1007/s00449-006-0067-y. Epub 2006 Jun 27.
Enzymatic hydrolysis of penicillin G by immobilized penicillin acylase in a nonionic surfactant mediated cloud point system was presented. The effect of the operation parameters on equilibrium pH of this enzymatic hydrolysis process without pH control was examined. A relatively high equilibrium pH in cloud point system without pH control can be obtained. The feasibility of recycling utilization of the nonionic surfactant, a novel green solvent, was also investigated experimentally. Enzymatic hydrolysis of penicillin G in a discrete semi-batch mode, which simulates a semi-continuous process, envisages a completely eco-friendly, sustainable and efficient process for production of 6-aminopenicillanic acid.
