Cortes Isabel, Molinari Jose Luis, Solano Sandra, Hernandez-Mendoza Lilian, Ramirez Antonio, Tato Patricia
Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, 04510 Mexico City, Mexico.
Parasitol Res. 2003 Jul;90(4):273-9. doi: 10.1007/s00436-003-0855-0. Epub 2003 Mar 26.
The purpose of this study was to determine the Th1 and Th2 cytokine responses induced by Taenia solium metacestode antigens in mice and correlate them with the immune responses elicited in vivo. To assess this aim, mice were inoculated with metacestode antigens. RNA was obtained from spleen cells of immunized or control mice incubated with metacestode antigens and used to determine the cytokine profile. Peripheral blood eosinophilia was measured daily in each mouse and specific serum antibody levels were determined. Results showed that metacestode antigens induce the synthesis of IL-4, IL-5 and IFN-gamma mRNAs in spleen cells. They also induced peripheral blood eosinophilia and elicited specific IgE and IgG antibodies, especially IgG1. Three antigens were recognized by all IgG subclasses and by IgE (104, 88 and 7 kDa), and a 57-kDa protein was recognized by IgG1, IgG2a, IgG2b, and IgE. IgG1 and IgG2b recognized 52, 30 and 20 kDa antigens. Immune responses elicited in vivo and the cytokine profile showed good correlation.
本研究的目的是确定猪带绦虫囊尾蚴抗原在小鼠体内诱导的Th1和Th2细胞因子反应，并将其与体内引发的免疫反应相关联。为评估这一目标，给小鼠接种囊尾蚴抗原。从与囊尾蚴抗原孵育的免疫小鼠或对照小鼠的脾细胞中获取RNA，用于确定细胞因子谱。每天测量每只小鼠外周血嗜酸性粒细胞增多情况，并测定特异性血清抗体水平。结果显示，囊尾蚴抗原可诱导脾细胞合成IL-4、IL-5和IFN-γ mRNA。它们还诱导外周血嗜酸性粒细胞增多，并引发特异性IgE和IgG抗体，尤其是IgG1。所有IgG亚类和IgE均识别三种抗原（104、88和7 kDa），IgG1、IgG2a、IgG2b和IgE识别一种57-kDa蛋白。IgG1和IgG2b识别52、30和20 kDa抗原。体内引发的免疫反应与细胞因子谱显示出良好的相关性。
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