Guo Ying-Jun, Sun Shu-Han, Zhang Yi, Chen Zhu-Huan, Wang Kai-Yu, Huang Li, Zhang Shu, Zhang Hong-Ying, Wang Qing-Min, Wu Dan, Zhu Wei-Jia
Department of Medical Genetics, The Second Military Medical University, Shanghai 200433, China.
Vaccine. 2004 Sep 28;22(29-30):3841-7. doi: 10.1016/j.vaccine.2004.07.012.
In the present study, we investigated the duration of protection afforded to pigs immunized in two different prime-boost regimens: one is homologus priming and boosting with a protein vaccine, and the other is priming with a DNA vaccine and boosting with the protein vaccine. Groups of pigs that received the same vaccination regimen were then challenged with Taenia solium eggs at 6, 12 or 20 weeks post-immunization (wpi), respectively. The results showed that all vaccinated pigs challenged at 6 or 12 wpi showed significant (P < 0.05) reduction in the development of cysts. When challenged at 20 wpi, pigs primed with the DNA vaccine (pcDNA3-cC1) followed by two boosters of the protein vaccine (GST-cC1) showed significant (P < 0.05) protection against the challenge of T. solium eggs, whereas pigs receiving three injections of the protein vaccine showed no significant protection compared to non-vaccinated controls (P > 0.05). Antibody isotype assays showed that DNA prime-protein boost regimen induced a predominantly IgG2 response, compared to an IgG1 biased response for the protein prime-protein boost regimen. In addition, peripheral blood mononuclear cells (PBMC) obtained from the DNA prime-protein boost group proliferated strongly in response to GST-cC1 protein, and this responsiveness persisted until 20 wpi. Taken together, our data suggest that the use of a prime-boost strategy combining DNA and protein vaccines may be better than protein alone for the longevity of protection against the challenge of T. solium eggs.
在本研究中，我们调查了两种不同的初免-加强免疫方案对猪的保护持续时间：一种是用蛋白质疫苗进行同源初免和加强免疫，另一种是用DNA疫苗初免并用蛋白质疫苗加强免疫。然后，分别在免疫后6周、12周或20周，用猪带绦虫卵对接受相同疫苗接种方案的猪群进行攻毒。结果显示，在6周或12周攻毒的所有接种疫苗的猪，其囊肿发育均显著（P<0.05）减少。在20周攻毒时，先用DNA疫苗（pcDNA3-cC1）初免，然后用蛋白质疫苗（GST-cC1）进行两次加强免疫的猪，对猪带绦虫卵攻毒显示出显著（P<0.05）的保护作用，而接受三次蛋白质疫苗注射的猪与未接种疫苗的对照组相比，未显示出显著的保护作用（P>0.05）。抗体亚型分析表明，与蛋白质初免-蛋白质加强免疫方案诱导的以IgG1为主的反应相比，DNA初免-蛋白质加强免疫方案诱导的主要是IgG2反应。此外，从DNA初免-蛋白质加强免疫组获得的外周血单个核细胞（PBMC）对GST-cC1蛋白有强烈增殖反应，且这种反应性持续到20周。综上所述，我们的数据表明，对于抵抗猪带绦虫卵攻毒的长期保护而言，使用DNA疫苗和蛋白质疫苗联合的初免-加强免疫策略可能比单独使用蛋白质疫苗更好。
