Hoh Y K, Tan T K, Yeoh H H
Department of Botany, Faculty of Science, National University of Singapore.
Appl Biochem Biotechnol. 1992 Oct;37(1):81-8. doi: 10.1007/BF02788859.
Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2) - 9.1 x 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and beta-glucosidase activities. One segregant showed a 2.5-fold increase in beta-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved beta-glucosidase activities.
在聚乙二醇和钙离子的诱导下，对两株营养缺陷型黑曲霉进行了原生质体融合。融合频率在6.2×10⁻² - 9.1×10⁻²之间。诱导二倍体单倍体化后，分离出了显示亲本遗传标记组合的各种分离子。与二倍体不同，单倍体分离子在形态和β-葡萄糖苷酶活性方面表现出更大的变异。一个分离子的β-葡萄糖苷酶活性比亲本高2.5倍。因此，该方法对于创建具有更高β-葡萄糖苷酶活性的新型重组黑曲霉菌株似乎很有前景。
