Wang Zheng-rong, Bao Huai-en
Department of Laboratory Medicine, Guiyang Medical College, Guiyang 550004.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(1):20-3.
To identify the types of Taenia saginata isolated from Dali of Yunnan Province, and from Duyun and Congjiang of Guizhou Province.
Genomic DNA was isolated, and the mitochondrial cytochrome C oxidase subunit I (mtCO I) genes were amplified by polymerase chain reaction(PCR) and analyzed by PHYLIP software package.
The mtCO I gene sequences of Lanping sample were identical to that of T. saginata asiatica known in Taiwan, and the samples obtained from Dali and Duyun showed the same mtCO I gene sequences, while the sample from Congjiang had the same mtCO I gene sequences as T. saginata. The homology between these two groups of gene sequences was 97.44%, while the homology of amino acid sequences reached to 99.16%. The constructed phylogenetic tree revealed that the relationship between T. saginata asiatica and T. saginata is closer, both are distant relative to T. solium and other species of cestodes.
The Taenia prevalent in Lanping, Dali and Duyun is identified as T. saginata asiatica, while that isolated in Congjiang is the typical T. saginata.
鉴定从云南省大理市以及贵州省都匀市和从江县分离出的牛带绦虫种类。
提取基因组DNA，采用聚合酶链反应（PCR）扩增线粒体细胞色素C氧化酶亚基I（mtCO I）基因，并使用PHYLIP软件包进行分析。
兰坪样本的mtCO I基因序列与台湾已知的亚洲牛带绦虫相同，大理和都匀的样本显示出相同的mtCO I基因序列，而从江的样本与牛带绦虫具有相同的mtCO I基因序列。这两组基因序列之间的同源性为97.44%，而氨基酸序列的同源性达到99.16%。构建的系统发育树表明，亚洲牛带绦虫和牛带绦虫之间的关系更近，两者相对于猪带绦虫和其他绦虫种类都有较远的亲缘关系。
兰坪、大理和都匀流行的牛带绦虫被鉴定为亚洲牛带绦虫，而从江分离出的是典型的牛带绦虫。
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