Jeon Hyeong-Kyu, Kim Kyu-Heon, Eom Keeseon S
Department of Parasitology and Medical Research Institute, Chungbuk National University College of Medicine, Gaeshin-Dong, Chongju, Chungbuk 361-763, South Korea.
Parasitol Int. 2007 Sep;56(3):243-6. doi: 10.1016/j.parint.2007.04.001. Epub 2007 Apr 19.
The complete sequence of the Taenia saginata mitochondrial genome was determined, and its organization and structure were compared to other human-tropic Taenia tapeworms for which complete mitochondrial sequence data were available. The mitochondrial genome was 13,670 bp long, contained 12 protein-coding genes, two ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). It did not encode the atp8 gene. Overlapping regions were found between nad4L and nad4, nad1 and trnN, and cox1 and trnT. The ATG initiation codon was used for 10 protein-coding genes, and the GTG initiation codon was used for the remaining 2 genes (nad4 and atp6). The size of the protein-coding genes of the three human Taenia tapeworms did not vary, except for Taenia solium nad1 (891 aa) and nad4 (1212 aa) and Taenia asiatica cox2 (576 aa). The tRNA genes were 57-75 bp long, and the predicted secondary structures of 18 of these genes had typical clover-leaf shapes with paired dihydrouridine (DHU) arms. The genes in all human Taenia tapeworms for the two mitochondrial rRNA subunits rrnL and rrnS are separated by trnC. The putative T. saginata rrnL and rrnS are 972 and 732 bp long, respectively. The non-coding regions of the mt genome of T. saginata consisted of 2 regions: a short non-coding region (SNR, 66 nucleotides) and a long non-coding region (LNR, 159 nucleotides). The overall sequence difference in the full mitochondrial genome between T. saginata and T. asiatica was 4.6%, while T. solium differed by 11%. In conclusion, the complete sequence of the T. saginata mitochondrial genome will serve as a resource for comparative mitochondrial genomics and systematic studies of the parasitic cestodes.
测定了牛带绦虫线粒体基因组的完整序列，并将其组织和结构与其他有完整线粒体序列数据的人体寄生带绦虫进行了比较。线粒体基因组长度为13670 bp，包含12个蛋白质编码基因、两个核糖体RNA（rRNA，一个小亚基和一个大亚基）以及22个转运RNA（tRNA）。它不编码atp8基因。在nad4L与nad4、nad1与trnN以及cox1与trnT之间发现了重叠区域。10个蛋白质编码基因使用ATG起始密码子，其余2个基因（nad4和atp6）使用GTG起始密码子。三种人体带绦虫的蛋白质编码基因大小没有变化，但猪带绦虫的nad1（891个氨基酸）和nad4（1212个氨基酸）以及亚洲带绦虫的cox2（576个氨基酸）除外。tRNA基因长度为57 - 75 bp，其中18个基因的预测二级结构具有典型的三叶草形状，带有配对的二氢尿嘧啶（DHU）臂。所有人体带绦虫中，两个线粒体rRNA亚基rrnL和rrnS的基因被trnC隔开。推测的牛带绦虫rrnL和rrnS分别长972 bp和732 bp。牛带绦虫线粒体基因组的非编码区由2个区域组成：一个短非编码区（SNR，66个核苷酸）和一个长非编码区（LNR，159个核苷酸）。牛带绦虫和亚洲带绦虫整个线粒体基因组的总体序列差异为4.6%，而与猪带绦虫的差异为11%。总之，牛带绦虫线粒体基因组的完整序列将为寄生绦虫的比较线粒体基因组学和系统研究提供资源。
