Risatti Guillermo R, Pomp Daniel, Donis Ruben O
Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska 68583-0905, USA.
Anim Biotechnol. 2003 May;14(1):31-49. doi: 10.1081/ABIO-120020184.
Bovine viral diarrhea virus (BVDV) infection in cattle is responsible for mucosal disease; an invariably fatal syndrome characterized by the recovery of two BVDV strains: cytopathic (cp) or noncytopathic (ncp). To understand the cellular responses to cp BVDV infection, we carried out differential display-polymerase chain reaction (DD-PCR) analysis of gene expression in infected cells. Altered expression of 14 genes involved in several functions was observed in cells infected with cp BVDV: (1) immune regulation, such as CD46, FKBP-12, and osteopontin (OPN); (2) apoptosis-related cysteine proteases like calpain; (3) signaling plasma membrane proteins such as integrin beta1, and prion protein; and (4) unknown function genes. Northern blot analysis of the expression of these genes in ncp BVDV infected cells revealed that while the expression of some genes was affected as in cp BVDV infected cells, others show a clearly contrary change. We postulate that a cause-effect relationship may exist between the differential gene expression alterations that characterize cp and ncp BVDV infections and the unique diseases associated with each BVDV biotype.
牛病毒性腹泻病毒（BVDV）感染牛会引发黏膜病；这是一种必然致命的综合征，其特征是出现两种BVDV毒株：细胞病变型（cp）或非细胞病变型（ncp）。为了解细胞对cp BVDV感染的反应，我们对感染细胞中的基因表达进行了差异显示聚合酶链反应（DD-PCR）分析。在感染cp BVDV的细胞中观察到14个参与多种功能的基因表达发生了改变：（1）免疫调节相关基因，如CD46、FKBP-12和骨桥蛋白（OPN）；（2）凋亡相关的半胱氨酸蛋白酶，如钙蛋白酶；（3）信号转导质膜蛋白，如整合素β1和朊病毒蛋白；（4）功能未知的基因。对这些基因在感染ncp BVDV细胞中的表达进行Northern印迹分析发现，虽然一些基因的表达与感染cp BVDV的细胞一样受到影响，但其他基因则呈现出明显相反的变化。我们推测，cp和ncp BVDV感染所特有的差异基因表达改变与每种BVDV生物型相关的独特疾病之间可能存在因果关系。
