Scanlan Perry M, Tiwari Vaibhav, Bommireddy Susmita, Shukla Deepak
Department of Microbiology-Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
Virology. 2003 Jul 20;312(1):14-24. doi: 10.1016/s0042-6822(03)00176-4.
Entry of herpes simplex virus-1 (HSV-1) into cells requires a concerted action of four viral glycoproteins gB, gD, and gH-gL. Previously, cell surface expression of gD had been shown to confer resistance to HSV-1 entry. To investigate any similar effects caused by other entry glycoproteins, gB and gH-gL were coexpressed with Nectin-1 in Chinese hamster ovary (CHO) cells. Interestingly, cellular expression of gB had no effect on HSV-1(KOS) entry. In contrast, entry was significantly reduced in cells expressing gH-gL. This effect was further analyzed by expressing gH and gL separately. Cells expressing gL were normally susceptible, whereas gH-expressing cells were significantly resistant. Further experiments suggested that the gH-mediated interference phenomenon was not specific to any particular gD receptor and was also observed in gH-expressing HeLa cells. Moreover, contrary to a previous report, gL-independent cell surface expression of gH was detected in stably transfected CHO cells, possibly implicating cell surface gH in the interference phenomenon. Thus, taken together these findings indicate that cellular expression of gH interferes with HSV-1 entry.
单纯疱疹病毒1型（HSV-1）进入细胞需要四种病毒糖蛋白gB、gD和gH-gL协同作用。此前，已证明gD在细胞表面表达可赋予细胞对HSV-1进入的抗性。为了研究其他进入糖蛋白是否会产生类似效应，将gB和gH-gL与Nectin-1在中国仓鼠卵巢（CHO）细胞中共表达。有趣的是，gB在细胞中的表达对HSV-1（KOS）进入没有影响。相反，在表达gH-gL的细胞中，病毒进入显著减少。通过分别表达gH和gL进一步分析了这种效应。表达gL的细胞通常易感，而表达gH的细胞则显著抗性。进一步的实验表明，gH介导的干扰现象并非特定于任何特定的gD受体，在表达gH的HeLa细胞中也观察到了这种现象。此外，与之前的报道相反，在稳定转染的CHO细胞中检测到了不依赖gL的gH细胞表面表达，这可能意味着细胞表面的gH参与了干扰现象。因此，综合这些发现表明，gH在细胞中的表达会干扰HSV-1的进入。
