Roess Deborah A, Smith Steven M L
Department of Biomedical Sciences and Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, Colorado 80523, USA.
Biol Reprod. 2003 Dec;69(6):1765-70. doi: 10.1095/biolreprod.103.018846. Epub 2003 Jul 30.
Membrane motions of LH receptors following binding of hormone agonists are consistent with hormone-driven aggregation. It is increasingly apparent that G protein-coupled receptors, including the LH receptor, are engaged in dynamic interactions with one another and other membrane components. These interactions are governed, in part, by a number of factors including whether the receptor has bound ligand, whether the receptor is capable of transducing a hormone-mediated signal, and the nature of the membrane environment within which the receptor is found. Microscopic methods, including laser-optical techniques, are ideally suited to probe dynamic events on cell membranes and provide an opportunity to examine interactions between receptors and other membrane components on viable cells. We and others have used a variety of techniques, some of which are summarized below, to examine functional and nonfunctional LH receptors on viable cells and the membrane environment of these receptors during cell signaling events.
激素激动剂结合后促黄体生成素受体的膜运动与激素驱动的聚集一致。越来越明显的是，包括促黄体生成素受体在内的G蛋白偶联受体彼此之间以及与其他膜成分进行动态相互作用。这些相互作用部分受多种因素支配，包括受体是否结合了配体、受体是否能够转导激素介导的信号以及受体所处的膜环境的性质。包括激光光学技术在内的微观方法非常适合探测细胞膜上的动态事件，并为研究活细胞上受体与其他膜成分之间的相互作用提供了机会。我们和其他人已经使用了多种技术（其中一些总结如下）来研究活细胞上功能性和非功能性促黄体生成素受体以及这些受体在细胞信号传导事件中的膜环境。
