Wiltfang Jens, Esselmann Hermann, Smirnov Alexander, Bibl Mirko, Cepek Lukas, Steinacker Petra, Mollenhauer Brit, Buerger Katharina, Hampel Harald, Paul Sabine, Neumann Manuela, Maler Manuel, Zerr Inga, Kornhuber Johannes, Kretzschmar Hans A, Poser Sigrid, Otto Markus
Department of Psychiatry, University Erlangen-Nürnberg, Germany.
Ann Neurol. 2003 Aug;54(2):263-7. doi: 10.1002/ana.10661.
Decreased levels of beta-amyloid peptide 1-42 (Abeta1-42) in cerebrospinal fluid (CSF) are a characteristic feature of Alzheimer's disease (AD) but recently were also observed in Creutzfeldt-Jakob disease (CJD). We analyzed the CSF of patients with CJD, and AD and nondemented controls using a quantitative urea-based Abeta sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblot. Like in AD and nondemented controls, we found a highly conserved pattern of carboxyterminally truncated Abeta1-37/38/39 in addition to Abeta1-40/42 also in CJD patients. By the introduction of the ratio Abeta1-39 to Abeta1-42, CJD and AD can effectively be differentiated. We conclude that the immunoblot shows disease-specific CSF Abeta peptide patterns in CJD and AD and suppose that measurement of the Abeta peptide pattern seems to be a promising diagnostic tool in the differential diagnosis of dementias.
脑脊液（CSF）中β-淀粉样肽1-42（Aβ1-42）水平降低是阿尔茨海默病（AD）的一个特征性表现，但最近在克雅氏病（CJD）中也观察到了这一现象。我们使用基于尿素的Aβ十二烷基硫酸钠聚丙烯酰胺凝胶电泳免疫印迹法，对CJD、AD患者以及非痴呆对照者的脑脊液进行了分析。与AD患者和非痴呆对照者一样，我们在CJD患者中除了发现Aβ1-40/42外，还发现了羧基末端截短的Aβ1-37/38/39的高度保守模式。通过引入Aβ1-39与Aβ1-42的比值，可以有效地区分CJD和AD。我们得出结论，免疫印迹显示了CJD和AD患者脑脊液中疾病特异性的Aβ肽模式，并推测Aβ肽模式的检测似乎是痴呆症鉴别诊断中有前景的诊断工具。
