Smirnov E B, Puchkov V F
Department of Morphology, RAMS Institute of Experimental Medicine, St. Petersburg.
Morfologiia. 2003;123(2):51-6.
The retinal primordia were studied in human embryos at developmental weeks 5-8 to examine the expression of proliferation-associated proteins (Ki67 and PCNA), the number of nucleoli in the nuclei of neuroepithelial cells (in respect to their distance to the apical surface) and the distribution of DNA-synthesizing cells after a short-term (20 min) incubation in vitro in serum-free medium containing BrdU. In 5-week embryos, retinal primordium contains the neuroepithelium with a typical structure. BrdU-positive nuclei and nuclei with small number of nucleoli were found in the basal portion of the ventricular zone. However, this organization was disturbed in the initial period of the formation of inner nuclear layer (week 6). At this stage DNA-synthesizing cells were seen even at the apical surface. In retinal primordia of embryos at weeks 6-7 there appeared an additional area of cell proliferation in the Chievitz layer and in the inner nuclear layer. In embryos at week 8 the dividing cells were concentrated in the outer nuclear layer, which has regained the organization, typical for a neuroepithelium.
在发育第5 - 8周的人类胚胎中研究视网膜原基，以检查增殖相关蛋白（Ki67和PCNA）的表达、神经上皮细胞核中核仁的数量（相对于它们到顶端表面的距离）以及在含有BrdU的无血清培养基中体外短期（20分钟）孵育后DNA合成细胞的分布。在5周龄的胚胎中，视网膜原基包含具有典型结构的神经上皮。在室管膜区的基部发现了BrdU阳性细胞核和核仁数量少的细胞核。然而，在内核层形成的初期（第6周），这种组织结构受到了干扰。在这个阶段，甚至在顶端表面也能看到DNA合成细胞。在6 - 7周龄胚胎的视网膜原基中，在Chievitz层和内核层出现了一个额外的细胞增殖区域。在8周龄的胚胎中，分裂细胞集中在外核层，该层已经恢复了神经上皮典型的组织结构。
