van der Maas Peter, van de Sandt Thomas, Klapwijk Bram, Lens Piet
Sub-Department of Environmental Technology, Wageningen University, "Biotechnion"-Bomenweg 2, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
Biotechnol Prog. 2003 Jul-Aug;19(4):1323-8. doi: 10.1021/bp030008n.
The reduction of nitric oxide (NO) in aqueous solutions of Fe(II)EDTA is one of the core processes in BioDeNOx, an integrated physicochemical and biological technique for NO(x)() removal from industrial flue gases. NO reduction in aqueous solutions of Fe(II)EDTA (20-25 mM, pH 7.2 +/- 0.2) was investigated in batch experiments at 55 degrees C. Reduction of NO to N(2) was found to be biologically catalyzed with nitrous oxide (N(2)O) as an intermediate. Various sludges from full-scale denitrifying and anaerobic reactors were capable to catalyze NO reduction under thermophilic conditions. The NO reduction rate was not affected by the presence of ethanol or acetate. EDTA-chelated Fe(II) was found to be a suitable electron donor for the biological reduction of nitric oxide to N(2), with the concomitant formation of Fe(III)EDTA. In the presence of ethanol, EDTA-chelated Fe(III) was reduced to Fe(II)EDTA. This study strongly indicates that redox cycling of FeEDTA plays an important role in the biological denitrification process within the BioDeNOx concept.
在用于从工业烟道气中去除氮氧化物的综合物理化学和生物技术BioDeNOx中，Fe(II)EDTA水溶液中一氧化氮（NO）的还原是核心过程之一。在55℃下进行的批次实验中，研究了Fe(II)EDTA（20 - 25 mM，pH 7.2±0.2）水溶液中的NO还原。发现NO还原为N₂是由生物催化的，以一氧化二氮（N₂O）作为中间产物。来自全尺寸反硝化和厌氧反应器的各种污泥能够在嗜热条件下催化NO还原。NO还原速率不受乙醇或乙酸盐存在的影响。发现EDTA螯合的Fe(II)是将一氧化氮生物还原为N₂的合适电子供体，同时形成Fe(III)EDTA。在乙醇存在下，EDTA螯合的Fe(III)被还原为Fe(II)EDTA。这项研究强烈表明，FeEDTA的氧化还原循环在BioDeNOx概念中的生物反硝化过程中起着重要作用。
