Clausen Mads H, Willats William G T, Knox J Paul
Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UK.
Carbohydr Res. 2003 Aug 12;338(17):1797-800. doi: 10.1016/s0008-6215(03)00272-6.
A range of synthetic methyl hexagalacturonates were used as potential hapten inhibitors in competitive-inhibition enzyme-linked immunosorbent assays (ELISAs) with anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. The selective inhibition of these antibodies by different haptens provides insight into the structures of the partially methyl-esterified pectin epitopes of these widely used monoclonal antibodies.
一系列合成甲基六半乳糖醛酸酯被用作潜在的半抗原抑制剂，用于与抗同型半乳糖醛酸单克隆抗体LM7、JIM5和JIM7进行的竞争抑制酶联免疫吸附测定（ELISA）。不同半抗原对这些抗体的选择性抑制为深入了解这些广泛使用的单克隆抗体的部分甲基酯化果胶表位结构提供了线索。
