Maebuchi Motohiro, Machidori Mika, Urade Reiko, Ogawa Tadashi, Moriyama Tatsuya
Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Arch Biochem Biophys. 2003 Aug 15;416(2):164-70. doi: 10.1016/s0003-9861(03)00279-0.
Obesity is a major risk factor for insulin resistance. Resistin, an adipocyte-derived hormone-like molecule, is considered to serve as an important link between obesity and insulin resistance. However, the physiological role of resistin and the mechanism by which it neutralizes insulin action are still unclear. There are also conflicting reports that cast doubt on the cause of insulin resistance. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) system for quantification of mouse resistin levels, analyzed in relation to insulin resistance. C57BL/6J mice fed high-fat diet compared with normal diet had low resistin levels (by 70%, P<0.01) in epididymal adipose tissues. Genetically obese mice, db/db and KK-A(y), had hyperinsulinemia and hyperglycemia but low resistin levels (decreases by 83 and 90%, both P<0.01) compared with C57/BL6J mice in epididymal adipose tissues. Serum resistin levels determined by Western blotting showed a similar pattern to those in adipose tissues. Resistin levels in adipose tissues correlated with serum adiponectin concentrations positively (r=0.49). Our results indicate that the novel ELISA system is suitable for measurement of resistin levels in adipose tissues. The results do not support a role for resistin in insulin resistance.
肥胖是胰岛素抵抗的主要危险因素。抵抗素是一种脂肪细胞衍生的类激素分子，被认为是肥胖与胰岛素抵抗之间的重要联系。然而，抵抗素的生理作用及其中和胰岛素作用的机制仍不清楚。也有相互矛盾的报道对胰岛素抵抗的原因提出质疑。在本研究中，我们开发了一种酶联免疫吸附测定（ELISA）系统用于定量小鼠抵抗素水平，并分析其与胰岛素抵抗的关系。与正常饮食喂养的C57BL/6J小鼠相比，高脂饮食喂养的小鼠附睾脂肪组织中的抵抗素水平较低（降低70%，P<0.01）。基因肥胖小鼠db/db和KK-A(y)存在高胰岛素血症和高血糖，但与C57/BL6J小鼠相比，其附睾脂肪组织中的抵抗素水平较低（分别降低83%和90%，均P<0.01）。通过蛋白质印迹法测定的血清抵抗素水平与脂肪组织中的水平呈现相似模式。脂肪组织中的抵抗素水平与血清脂联素浓度呈正相关（r=0.49）。我们的结果表明，这种新型ELISA系统适用于测量脂肪组织中的抵抗素水平。这些结果不支持抵抗素在胰岛素抵抗中起作用。
