抗犬红细胞抗原1.1鼠单克隆抗体的制备、特性鉴定及应用
Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1.
作者信息
Andrews G A, Chavey P S, Smith J E
机构信息
Department of Veterinary Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506-5605.
出版信息
J Am Vet Med Assoc. 1992 Nov 15;201(10):1549-52.
A murine IgM monoclonal antibody, which recognizes dog erythrocyte antigen (DEA) 1.1, has been produced. The antibody correctly identified canine RBC possessing DEA 1.1 in a panel of RBC typed by an independent laboratory. Reactivity of the monoclonal antibody was compared with canine anti-DEA 1.1 antiserum with 163 RBC samples from 145 dogs. Results of agglutination tests with the 2 reagents were in agreement for all samples. A card agglutination test that uses the monoclonal antibody with blood is described. A monoclonal antibody-based test should facilitate blood typing for DEA 1.1 in clinical practice.
已制备出一种识别犬红细胞抗原（DEA）1.1的鼠源IgM单克隆抗体。在一个由独立实验室进行红细胞分型的样本组中，该抗体正确识别了具有DEA 1.1的犬红细胞。将该单克隆抗体的反应性与犬抗DEA 1.1抗血清针对来自145只犬的163个红细胞样本进行了比较。两种试剂的凝集试验结果对所有样本均一致。描述了一种使用该单克隆抗体进行血液卡片凝集试验的方法。基于单克隆抗体的检测方法应有助于临床实践中DEA 1.1的血型鉴定。
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