Du Xiangying, Yang Yuesong, Le Visage Catherine, Chen Hunter H, DeJong Robert, Qiu Bensheng, Wang Danming, Leong Kam W, Hamper Ulrike M, Yang Xiaoming
Departments of Radiology and Biomedical Engineering, Johns Hopkins University School of Medicine, 720 Rutland Ave, Traylor Bldg, Rm 330, Baltimore, MD 21205, USA.
Radiology. 2003 Aug;228(2):555-9. doi: 10.1148/radiol.2282020539.
In this study, the authors tested the feasibility of using ultrasonography (US) to monitor catheter-based vascular gene microsphere delivery. Polymeric biodegradable microspheres (mean diameter, 5 microm) were prepared by using a double-emulsion technique to encapsulate DNA-plasmid-encoding green fluorescent protein (GFP) genes. With use of gene-delivery catheters, GFP microspheres were locally delivered into the left femoral arterial walls of six pigs; the contralateral arteries were not infused with microspheres and thus served as negative control vessels. The delivery procedures were monitored with high-frequency (8-15-MHz) transducer US. The effectiveness of monitoring with US was compared with the effectiveness of monitoring with immunohistochemical anti-GFP staining. A highly echogenic "star burst" sign around the entire vessel wall was seen at US and correlated with immunohistochemical findings that showed the destination of the gene microspheres. Study results demonstrate the potential of US for monitoring catheter-based vascular gene microsphere delivery in vivo.
在本研究中，作者测试了使用超声检查（US）监测基于导管的血管基因微球递送的可行性。通过使用双乳液技术制备聚合物可生物降解微球（平均直径5微米），以包封编码绿色荧光蛋白（GFP）基因的DNA质粒。使用基因递送导管，将GFP微球局部递送至6头猪的左股动脉壁；对侧动脉未注入微球，因此用作阴性对照血管。使用高频（8 - 15MHz）换能器超声监测递送过程。将超声监测的有效性与免疫组织化学抗GFP染色监测的有效性进行比较。在超声检查中可见整个血管壁周围有高回声的“星爆”征，且与显示基因微球归宿的免疫组织化学结果相关。研究结果证明了超声在体内监测基于导管的血管基因微球递送的潜力。
