Sánchez-Mendoza M Alicia, Martínez-Ayala Sonia O, Hernández-Hernández José A, Zúñiga-Sosa Leonor, Pastelín-Hernández Gustavo, Escalante-Acosta Bruno A
Departamento de Farmacología, Instituto Nacional de Cardiología Ignacio Chávez, INCICH, Juan Badiano No. 1, Col. Sección XVI, Tlalpan 14080 México, D.F.
Arch Cardiol Mex. 2003 Apr-Jun;73(2):98-104.
Nitric oxide and cytochrome P450 arachidonic acid metabolites participate in blood pressure regulation. The synthesis of these autacoids leads to arterial hypertension. However, it is not known whether there is an interaction between them. Therefore, we studied the modulatory effect of nitric oxide and cytochrome P450-arachidonic acid metabolites, their interaction on blood pressure, and the renal content of cytochrome P450. Male Wistar rats were divided: 1) control, 2) L-NAME (100 mg/kg/d p.o.), 3) L-NAME + SnCl2 (10 mg/kg/d i.p.), and 4) L-NAME + dexamethasone (1 mg/kg/d s.c.). We measured blood pressure and collected urine and blood for nitric oxide measurement. NO2 was quantified by HPLC. Blood pressure was: control, 97 +/- 7 mmHg; L-NAME, 151 +/- 4.6 mmHg; L-NAME + SnCl2, 133 +/- 3 mmHg, and L-NAME + dexamethasone 152 +/- 4.5 mmHg. Urine nitrite concentration was: 1) 1.832 +/- 0.32, 2) 1.031 +/- 0.23, 3) 1.616 +/- 0.33, and 4) 1.244 +/- 0.33 mumol/mL, while the concentration in blood was: 1) 0.293 +/- 0.06, 2) 0.150 +/- 0.05, 3) 0.373 +/- 0.13, and 4) 0.373 +/- 0.07 mumol/mL. L-NAME + SnCl2 decreased cytochrome P450 renal content, and L-NAME + dexamethasone showed a similar response. In conclusion, both, nitric oxide and CYP-arachidonic acid metabolites play a role in the regulation of blood pressure. Nitric oxide also partially regulates renal cytochrome P450 content.
一氧化氮和细胞色素P450花生四烯酸代谢产物参与血压调节。这些自分泌物质的合成会导致动脉高血压。然而，它们之间是否存在相互作用尚不清楚。因此，我们研究了一氧化氮和细胞色素P450 - 花生四烯酸代谢产物的调节作用、它们对血压的相互作用以及细胞色素P450的肾含量。将雄性Wistar大鼠分为：1）对照组，2）L - 精氨酸甲酯（100毫克/千克/天，口服），3）L - 精氨酸甲酯 + 氯化亚锡（10毫克/千克/天，腹腔注射），4）L - 精氨酸甲酯 + 地塞米松（1毫克/千克/天，皮下注射）。我们测量了血压，并收集尿液和血液用于一氧化氮测量。通过高效液相色谱法对二氧化氮进行定量。血压分别为：对照组97±7毫米汞柱；L - 精氨酸甲酯组151±4.6毫米汞柱；L - 精氨酸甲酯 + 氯化亚锡组133±3毫米汞柱，L - 精氨酸甲酯 + 地塞米松组152±4.5毫米汞柱。尿亚硝酸盐浓度分别为：1）1.832±0.32、2）1.031±0.23、3）1.616±0.33和4）1.244±0.33微摩尔/毫升，而血液中的浓度分别为：1）0.293±0.06、2）0.150±0.05、3）0.373±0.13和4）0.373±0.07微摩尔/毫升。L - 精氨酸甲酯 + 氯化亚锡降低了细胞色素P450的肾含量，L -精氨酸甲酯 + 地塞米松表现出类似的反应。总之，一氧化氮和细胞色素P450 - 花生四烯酸代谢产物在血压调节中均起作用。一氧化氮还部分调节肾细胞色素P450含量。
