干细胞因子和c-kit在人恶性纤维组织细胞瘤细胞系（TNMY1）中的表达。
Expression of stem cell factor and c-kit in human malignant fibrous histiocytoma cell line (TNMY1).
作者信息
Nakatani Tetsuya, Marui Takashi, Yamamoto Tetsuji, Hitora Toshiaki, Akisue Toshihiro, Kawamoto Teruya, Nagira Keiko, Fujita Ikuo, Matsumoto Keiji, Yoshiya Shinichi, Kurosaka Masahiro
机构信息
Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Japan.
出版信息
Anticancer Res. 2003 May-Jun;23(3B):2329-33.
BACKGROUND
The expression of c-kit and/or its ligand, stem cell factor (SCF), has been related to tumor proliferation, in several tumor systems.
MATERIALS AND METHODS
We analyzed the expression of the SCF/its receptor (c-kit) mRNA and the production of soluble SCF in a human malignant fibrous histiocytoma (MFH) cell line (TNMY1).
RESULTS
Immunocytochemical analysis revealed that the TNMY1 cells were positive for both SCF and c-kit. Reverse transcriptase-polymerase chain reaction (RT-PCR) showed that the TNMY1 cell line expressed mRNA for SCF and c-kit. By using an enzyme-linked immunosorbent assay (ELISA), the TNMY1 cells were found to produce relatively high amounts of soluble SCF. However, the addition of soluble SCF to the TNMY1 cells did not alter the in vitro growth ability of the cells.
CONCLUSION
Our data showed that the MFH cells produced consistent amounts of SCF but did not demonstrate autocrine growth modulation. Thus, SCF secretion may have a paracrine activity in the growth of MFH cells.
背景
在多个肿瘤系统中，c-kit及其配体干细胞因子（SCF）的表达与肿瘤增殖有关。
材料与方法
我们分析了人恶性纤维组织细胞瘤（MFH）细胞系（TNMY1）中SCF及其受体（c-kit）mRNA的表达以及可溶性SCF的产生。
结果
免疫细胞化学分析显示TNMY1细胞SCF和c-kit均呈阳性。逆转录聚合酶链反应（RT-PCR）表明TNMY1细胞系表达SCF和c-kit的mRNA。通过酶联免疫吸附测定（ELISA）发现TNMY1细胞产生相对大量的可溶性SCF。然而，向TNMY1细胞中添加可溶性SCF并未改变细胞的体外生长能力。
结论
我们的数据表明MFH细胞产生恒定数量的SCF，但未表现出自分泌生长调节。因此，SCF分泌可能在MFH细胞生长中具有旁分泌活性。
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