Morimoto M, Tsujimura T, Kanakura Y, Kitamura Y, Matsuda H
Department of Pathology, Faculty of Medicine, Osaka University, Japan.
Am J Vet Res. 1998 Mar;59(3):363-6.
To determine expression of c-kit and stem cell factor (SCF) mRNA in liver specimens from healthy adult dogs and to investigate whether differentiation of mast cells in the liver of dogs is supported by the c-kit receptor tyrosine kinase/SCF system.
3 healthy adult Beagles.
The nucleic acid sequence of the canine c-kit fragment of the intracellular tyrosine kinase domain was determined. Magnitudes of c-kit and SCF mRNA expression in liver samples was determined by means of northern blot analysis, using probes based on canine sequences. To determine which cells were expressing c-kit and SCF mRNA, in situ hybridization was performed.
Expression of c-kit and SCF mRNA in liver samples was weak but appreciable. In situ hybridization revealed that c-kit and SCF mRNA expression was restricted to mast cells and plasma cells, respectively.
Expression of SCF mRNA was detected in liver from healthy adult dogs. The c-kit receptor tyrosine kinase/SCF system may, possibly in combination with other growth factor and receptor systems, be involved in proliferation and differentiation of liver mast cells in dogs.
测定健康成年犬肝脏标本中c-kit和干细胞因子（SCF）mRNA的表达，并研究c-kit受体酪氨酸激酶/SCF系统是否支持犬肝脏中肥大细胞的分化。
3只健康成年比格犬。
测定细胞内酪氨酸激酶结构域的犬c-kit片段的核酸序列。使用基于犬序列的探针，通过Northern印迹分析确定肝脏样本中c-kit和SCF mRNA的表达量。为了确定哪些细胞表达c-kit和SCF mRNA，进行了原位杂交。
肝脏样本中c-kit和SCF mRNA的表达较弱但可检测到。原位杂交显示，c-kit和SCF mRNA的表达分别局限于肥大细胞和浆细胞。
在健康成年犬的肝脏中检测到SCF mRNA的表达。c-kit受体酪氨酸激酶/SCF系统可能与其他生长因子和受体系统共同参与犬肝脏肥大细胞的增殖和分化。
