Vignoles P, Favennec L, Rondelaud D, Dreyfuss G
UPRES-EA no. 3174, Facultés de Pharmacie et de Médecine, 2 rue du Docteur Marcland, 87025 Cedex, France.
J Helminthol. 2003 Sep;77(3):275-7. doi: 10.1079/JOH2003177.
Laboratory investigations on three aquatic populations of Galba truncatula, originating from the Peruvian Altiplano and French Massif Central, were carried out during three successive snail generations to determine if these populations might be successfully used for the metacercarial production of Fasciola hepatica under experimental conditions. High numbers of surviving snails at day 30 post-exposure (>70%), high prevalences of F. hepatica infections (>60%), and prolonged productions of cercariae for a mean period of 35 to 47 days were observed in the three populations, whatever the snail generation. In the Peruvian population, metacercariae of F. hepatica significantly decreased in numbers from a mean of 251 in the parent snails to 124 per snail in the F2 generation, whereas no significant variation was observed in the two French populations. As these aquatic snails rarely emerged out of water, the use of these populations for the commercial production of F. hepatica metacercariae was of great interest, because the daily time spent watching the breeding boxes of snails was clearly shorter, thereby reducing the cost of producing metacercariae compared with using amphibious snails reared with romaine lettuce.
对源自秘鲁高原和法国中央高原的三个截口土蜗水生种群进行了实验室研究，研究在连续三代蜗牛中开展，以确定在实验条件下这些种群是否可成功用于肝片吸虫后尾蚴的生产。无论蜗牛代数如何，在这三个种群中均观察到暴露后30天存活蜗牛数量众多（>70%）、肝片吸虫感染率高（>60%）以及尾蚴平均持续产生35至47天。在秘鲁种群中，肝片吸虫后尾蚴数量从亲代蜗牛的平均251个显著减少至F2代的每只蜗牛124个，而在两个法国种群中未观察到显著变化。由于这些水生蜗牛很少出水，利用这些种群进行肝片吸虫后尾蚴的商业生产具有很大意义，因为观察蜗牛养殖箱每天所需的时间明显更短，从而与使用用生菜饲养的两栖蜗牛相比，降低了后尾蚴的生产成本。
