[分娩期女性子宫肌层中环氧化酶-2 mRNA的表达及其剪接变体的鉴定]
[Expression of cyclooxygenase-2 mRNA and identification of its spliceriant in human myometrium obtained from women in labor].
作者信息
Huang Yin-ping, Ye Du-yun, Wu Ping, Huang Yun-feng, Sun Wei, Ying Hong-gang, Pan Qian, Wan Jing-yuan, Yuan Ping, Zhang Dai-juan
机构信息
Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
出版信息
Zhonghua Fu Chan Ke Za Zhi. 2003 May;38(5):270-2.
OBJECTIVE
To investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splice variant of COX-2.
METHODS
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used for investigating the expression of COX-2. According to the sequence of rat COX-2 splice variant, the primers were designed and synthesized, then the splice variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced.
RESULTS
The results showed that (1) The Expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than those in labor. (2) A new band of COX-2 was obtained in myometrium from a woman in labor. The fragment includes an unspliced intron, which locates between exons 7 and 8.
CONCLUSION
COX-2 gene is not only expressed highly in human myometrium from women in labor, but also produced splicing variant by alternative splicing.
目的
研究环氧化酶-2（COX-2）在分娩和未分娩女性子宫下段中的表达，并鉴定COX-2的剪接变体。
方法
采用逆转录聚合酶链反应（RT-PCR）研究COX-2的表达。根据大鼠COX-2剪接变体的序列设计并合成引物，然后鉴定分娩女性子宫肌层中COX-2的剪接变体，克隆到载体中并测序。
结果
结果显示：（1）未分娩女性子宫肌层中COX-2 mRNA的表达低于分娩女性。（2）在一名分娩女性的子宫肌层中获得了一条新的COX-2条带。该片段包含一个未剪接的内含子，位于外显子7和8之间。
结论
COX-2基因不仅在分娩女性的子宫肌层中高表达，而且通过可变剪接产生剪接变体。
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