Yang Xin, Zheng Shu-rong, Chen Rong-jing, Chen Shu-ling, Fan Zheng-hong, Li Hui, Lu Yun-hua
Department of Obstetrics and Gynecology, First Hospital, Peking University, Beijing 100034, China.
Zhonghua Fu Chan Ke Za Zhi. 2003 May;38(5):273-6.
To evaluate the relationship between the peak bone mineral density (PBMD) and vitamin D receptor (VDR), estrogen receptor (ER) allelic variants in Beijing young women.
From March, 2000 to July, 2001, one hundred and fifty-nine young healthy women (25 - 37 years old) in Beijing were voluntarily enrolled in the study. (1) BMD were measured by dual energy X-ray absorptiometry (DXEA) at lumbar and hip. (2) The polymorphism of VDR and ER genes were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). (3) The relationship between BMD and polymorphism of VDR and ER genes were examined.
(1) Lumber BMD was positively correlated to height, weight and body mass index (BMI), whereas, the femoral neck BMD only to weight, and the other sites of hip BMD to BMI. (2) Although subjects with the VDR bb genotype had higher BMD than those with Bb genotype at lumber, femoral neck, inter and troch, no significant difference was found (P > 0.05). (3) In Ward triangle, subjects with ER PP genotype had significantly lower BMD than those in ER Pp and pp genotypes (P < 0.05). (4) Women with BbPP genotype combination had lower BMD levels at lumber and hip, and with bbPP and Bbpp genotypes combination significantly higher lumber BMD levels than BbPP genotype (P < 0.05). However, the differences of BMD among subjects with different VDR and ER genotypes became not significant after adjusting the confounder of body weight.
(1) Body weight and BMI play important roles to PBMD of Beijing women. (2) There was no significant difference of BMD levels between VDR genotypes at any site. (3) PvuII polymorphism of ER gene was associated with low Ward triangle BMD. (4) There was significant relationship between the combination of ER and VDR polymorphisms at lumbar and hip BMD. Our data suggest that genetic variation at the ER locus, singly and in relation to the VDR locus, may influence the attainment and maintenance of peak bone mass in young women.
评估北京年轻女性的峰值骨密度（PBMD）与维生素D受体（VDR）、雌激素受体（ER）基因多态性之间的关系。
2000年3月至2001年7月，159名北京年轻健康女性（25 - 37岁）自愿参加本研究。（1）采用双能X线吸收法（DXEA）测量腰椎和髋部的骨密度（BMD）。（2）通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测VDR和ER基因的多态性。（3）研究BMD与VDR和ER基因多态性之间的关系。
（1）腰椎骨密度与身高、体重和体重指数（BMI）呈正相关，而股骨颈骨密度仅与体重相关，髋部其他部位骨密度与BMI相关。（2）虽然VDR基因bb基因型受试者在腰椎、股骨颈、转子间和粗隆的骨密度高于Bb基因型受试者，但差异无统计学意义（P>0.05）。（3）在Ward三角区，ER基因PP基因型受试者的骨密度显著低于ER基因Pp和pp基因型受试者（P<0.05）。（4）BbPP基因型组合的女性在腰椎和髋部的骨密度水平较低，而bbPP和Bbpp基因型组合的女性腰椎骨密度水平显著高于BbPP基因型（P<0.05）。然而，在调整体重混杂因素后，不同VDR和ER基因型受试者之间的骨密度差异变得不显著。
（1）体重和BMI对北京女性的PBMD起重要作用。（2）任何部位VDR基因型之间的骨密度水平无显著差异。（3）ER基因的PvuII多态性与Ward三角区低骨密度有关。（4）ER和VDR基因多态性组合与腰椎和髋部骨密度之间存在显著关系。我们的数据表明，ER基因位点的遗传变异，单独或与VDR基因位点相关，可能影响年轻女性峰值骨量的获得和维持。
