鸟分枝杆菌细胞壁生物合成的突变分析
Mutational analysis of cell wall biosynthesis in Mycobacterium avium.
作者信息
Laurent Jean-Pierre, Hauge Kirsten, Burnside Kellie, Cangelosi Gerard
机构信息
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
出版信息
J Bacteriol. 2003 Aug;185(16):5003-6. doi: 10.1128/JB.185.16.5003-5006.2003.
Abstract
The cell wall of the environmental pathogen Mycobacterium avium is important to its virulence and intrinsic antimicrobial resistance. To identify genes involved in cell wall biosynthesis, "transposome" insertion libraries were screened for mutants with altered colony morphology on medium containing the lipoprotein stain Congo red. Nineteen such mutants were isolated and mapped, including 10 with insertions in a functional island of cell wall biosynthetic genes that spans approximately 40 kb of the M. avium genome.
摘要
环境病原体鸟分枝杆菌的细胞壁对其毒力和内在抗微生物耐药性至关重要。为了鉴定参与细胞壁生物合成的基因，利用“转座体”插入文库在含有脂蛋白染色剂刚果红的培养基上筛选菌落形态改变的突变体。分离并定位了19个这样的突变体，其中10个在一个跨越鸟分枝杆菌基因组约40 kb的细胞壁生物合成基因功能岛内有插入。
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