Rosenthal Eben L, Shreenivas Satya, Peters Glenn E, Grizzle William E, Desmond Renee, Gladson Candece L
Division of Otolarynology-Head and Neck Surgery, Comprehensive Cancer Center, University of Alabama at Birmingham, 35249, USA.
Laryngoscope. 2003 Aug;113(8):1406-10. doi: 10.1097/00005537-200308000-00027.
OBJECTIVES/HYPOTHESIS: Head and neck cancer tumor cell invasion is responsible for both local destruction and distant metastasis. Invasion is largely mediated by matrix metalloproteases that are thought to be induced by tumor cell derived extracellular matrix metalloprotease inducer (EMMPRIN) in surrounding fibroblasts. We hypothesize that EMMPRIN is overexpressed in laryngeal cancer.
Retrospective analysis of patients with supraglottic laryngeal cancer.
Total protein immunoblotting and immunohistochemical analysis of normal and malignant tissue were performed to determine EMMPRIN expression. EMMPRIN immunoreactivity in 33 patients was correlated with clinicopathological features and survival.
Whole-tissue lysates of tumors (n = 8) and metastatic lymph nodes (n = 2), but not normal skin (n = 8) or mucosa (n = 6), expressed significant amounts of EMMPRIN by immunoblotting. EMMPRIN membrane immunoreactivity (transmembrane EMMPRIN score) was associated with nodal positivity (P =.07), and it was a borderline significant predictor of survival (Hazards Ratio = 2.4; 95% CI, 0.88-6.55). As a categorical variable, higher transmembrane EMMPRIN score was associated with higher mortality.
The present study helps to establish EMMPRIN as a widely expressed protein in dysplastic mucosa and supraglottic laryngeal cancer, but not in normal epithelial counterparts.
目的/假设：头颈癌肿瘤细胞的侵袭导致局部破坏和远处转移。侵袭主要由基质金属蛋白酶介导，这些酶被认为是由肿瘤细胞衍生的细胞外基质金属蛋白酶诱导剂（EMMPRIN）在周围成纤维细胞中诱导产生的。我们假设EMMPRIN在喉癌中过度表达。
对声门上型喉癌患者进行回顾性分析。
对正常组织和恶性组织进行总蛋白免疫印迹和免疫组化分析，以确定EMMPRIN的表达。对33例患者的EMMPRIN免疫反应性与临床病理特征及生存率进行相关性分析。
通过免疫印迹法检测，肿瘤组织（n = 8）和转移性淋巴结组织（n = 2）的全组织裂解液中表达大量EMMPRIN，而正常皮肤组织（n = 8）和黏膜组织（n = 6）中未检测到。EMMPRIN膜免疫反应性（跨膜EMMPRIN评分）与淋巴结阳性相关（P = 0.07），是生存率的临界显著预测指标（风险比 = 2.4；95%置信区间，0.88 - 6.55）。作为分类变量，较高的跨膜EMMPRIN评分与较高的死亡率相关。
本研究有助于确定EMMPRIN是一种在发育异常黏膜和声门上型喉癌中广泛表达的蛋白，但在正常上皮组织中不表达。
