产生微菌素B17、D93、J25和L的野生型大肠杆菌；微菌素L产生和免疫基因的克隆。
Wild-type Escherichia coli producing microcins B17, D93, J25, and L; cloning of genes for microcin L production and immunity.
作者信息
Sablé S, Duarte M, Bravo D, Lanneluc I, Pons A M, Cottenceau G, Moreno F
机构信息
Laboratoire de génie protéique et cellulaire, Université de La Rochelle, Pôle Sciences, 17042 La Rochelle CEDEX 01, France.
出版信息
Can J Microbiol. 2003 May;49(5):357-61. doi: 10.1139/w03-047.
For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindIII-SalI fragment, which includes the Mcc V immunity gene, cvi.
首次分离并鉴定出一株能产生四种微菌素（Mcc），即B17、D93、J25和L，且对Mcc V具有免疫性的大肠杆菌菌株。分别克隆了编码Mcc B17、D93和L产生的每个基因簇。Mcc L的基因簇克隆在一个13.5 kb的HindIII - SalI片段内，该片段包含Mcc V免疫基因cvi。
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