微菌素B17产生及免疫的遗传决定因素的克隆与定位
Cloning and mapping of the genetic determinants for microcin B17 production and immunity.
作者信息
San Millan J L, Hernandez-Chico C, Pereda P, Moreno F
出版信息
J Bacteriol. 1985 Jul;163(1):275-81. doi: 10.1128/jb.163.1.275-281.1985.
Abstract
Plasmid pMccB17 (70 kilobases [kb]) codes for the production of microcin B17, a peptide that inhibits DNA synthesis, and for microcin B17 immunity. A BamHI-EcoRI fragment of 5.1 kb from pMccB17 was cloned into pBR322 in two steps. The resulting plasmid (pMM102) overproduced microcin B17 and expressed immunity against microcin. Mcc- and Mcc- Imm- mutants were isolated on plasmids pMccB17 and pMM102 by deleting various DNA fragments and by inserting different translocatable elements. Physical and phenotypic characterization of these mutants showed that a DNA region of 3.0 to 3.5 kb is required to produce microcin B17, whereas an adjacent region of about 1.0 kb is required to express microcin B17 immunity.
摘要
质粒pMccB17（70千碱基[kb]）编码微菌素B17的产生，微菌素B17是一种抑制DNA合成的肽，同时还编码微菌素B17免疫蛋白。来自pMccB17的一个5.1 kb的BamHI - EcoRI片段分两步克隆到pBR322中。所得质粒（pMM102）过量产生微菌素B17并表达对微菌素的免疫性。通过缺失各种DNA片段和插入不同的可转位元件，在质粒pMccB17和pMM102上分离出Mcc - 和Mcc - Imm - 突变体。对这些突变体的物理和表型特征分析表明，产生微菌素B17需要3.0至3.5 kb的DNA区域，而表达微菌素B17免疫性则需要约1.0 kb的相邻区域。
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