Somers Daryl J, Fedak George, Savard Marc
Agriculture and Agri-Food Canada-Cereal Research Centre, Winnipeg, Canada.
Genome. 2003 Aug;46(4):555-64. doi: 10.1139/g03-033.
Fusarium head blight of wheat is an extremely damaging disease, causing severe losses in seed yield and quality. The objective of the current study was to examine and characterize alternate sources of resistance to Fusarium head blight (FHB). Ninety-one F1-derived doubled haploid lines from the cross Triticum aestivum 'Wuhan-1' x Triticum aestivum 'Maringa' were examined for disease reaction to Fusarium graminearum by single-floret injection in replicated greenhouse trials and by spray inoculation in replicated field trials. Field and greenhouse experiments were also used to collect agronomic and spike morphology characteristics. Seed samples from field plots were used for deoxynivalenol (DON) determination. A total of 328 polymorphic microsatellite loci were used to construct a genetic linkage map in this population and together these data were used to identify QTL controlling FHB resistance, accumulation of DON, and agronomic and spike morphology traits. The analysis identified QTL for different types of FHB resistance in four intervals on chromosomes 2DL, 3BS, and 4B. The QTLs on 4B and 3BS proximal to the centromere are novel and not reported elsewhere. QTL controlling accumulation of DON independent of FHB resistance were located on chromosomes 2DS and 5AS. Lines carrying FHB resistance alleles on 2DL and 3BS showed a 32% decrease in disease spread after single-floret injection. Lines carrying FHB resistance alleles on 3BS and 4B showed a 27% decrease from the mean in field infection. Finally, lines carrying favourable alleles on 3BS and 5AS, showed a 17% reduction in DON accumulation. The results support a polygenic and quantitative mode of inheritance and report novel FHB resistance loci. The data also suggest that resistance to FHB infection and DON accumulation may be controlled, in part, by independent loci and (or) genes.
小麦赤霉病是一种极具破坏性的病害，会导致种子产量和质量严重损失。本研究的目的是检测和鉴定对小麦赤霉病（FHB）的替代抗性来源。在重复的温室试验中，通过单小花注射法，以及在重复的田间试验中，通过喷雾接种法，检测了由普通小麦‘武汉1号’×普通小麦‘马林加’杂交产生的91个F1衍生双单倍体系对禾谷镰刀菌的病害反应。田间和温室试验还用于收集农艺和穗形态特征。田间小区的种子样本用于脱氧雪腐镰刀菌烯醇（DON）测定。总共328个多态性微卫星位点用于构建该群体的遗传连锁图谱，这些数据共同用于鉴定控制FHB抗性、DON积累以及农艺和穗形态性状的QTL。分析在2DL、3BS和4B染色体的四个区间鉴定出了不同类型FHB抗性的QTL。位于4B和靠近着丝粒的3BS上的QTL是新发现的，在其他地方尚未见报道。控制与FHB抗性无关的DON积累的QTL位于2DS和5AS染色体上。在2DL和3BS上携带FHB抗性等位基因的品系在单小花注射后病害扩展减少了32%。在3BS和4B上携带FHB抗性等位基因的品系在田间感染中比平均值减少了27%。最后，在3BS和5AS上携带有利等位基因的品系DON积累减少了17%。结果支持多基因和数量遗传模式，并报道了新的FHB抗性位点。数据还表明，对FHB感染和DON积累的抗性可能部分由独立的位点和（或）基因控制。
