Utsumi R, Katayama S, Ikeda M, Igaki S, Nakagawa H, Miwa A, Taniguchi M, Noda M
Department of Agricultural Chemistry, Kinki University, Nara, Japan.
Nucleic Acids Symp Ser. 1992(27):149-50.
We have cloned and sequenced new Escherichia coli genes which belong to member of the family of environmentally responsive two-component system and named evgA and evgS because their amino acid sequences were found the most homologus to the Bordetella pertussis bvgA and bvgS. They were mapped at 51 min. and extending from 6B9 to 7G9 in the Kohara miniset library of the E. coli chromosome. In fact, both EvgA and EvgS proteins predicted from their DNA sequences were identified in the in vitro coupled transcription translation system. When the evgA and evgS were expressed on multiple copy plasmid in an envZ deletion strain, ompC expression was also regulated by temperature, MgSO4 and nicotinic acid, by which virulence of Bordetella pertussis is controlled via BvgA and BvgS. These results indicate that ompC expression was controlled by in vivo cross-talk via EvgA and EvgS which can work in E. coli the same way as BvgA and BvgS.
我们已经克隆并测序了新的大肠杆菌基因，这些基因属于环境响应双组分系统家族成员，命名为evgA和evgS，因为发现它们的氨基酸序列与百日咳博德特氏菌的bvgA和bvgS最为同源。它们位于51分钟处，在大肠杆菌染色体的小原文库中从6B9延伸至7G9。事实上，从它们的DNA序列预测的EvgA和EvgS蛋白都在体外偶联转录翻译系统中被鉴定出来。当evgA和evgS在envZ缺失菌株的多拷贝质粒上表达时，ompC的表达也受温度、硫酸镁和烟酸的调节，百日咳博德特氏菌的毒力就是通过BvgA和BvgS受其控制的。这些结果表明，ompC的表达是通过EvgA和EvgS在体内的相互作用来控制的，它们在大肠杆菌中的作用方式与BvgA和BvgS相同。
