采用三重聚合酶链反应和高效液相色谱法对胃癌中的c-myc基因进行定量分析。
Quantification of the c-myc gene in gastric carcinomas by the triplex polymerase chain reaction and high performance liquid chromatography.
作者信息
Yoshimura S, Shishikura A, Koido S, Ushigome S, Suemizu H, Taniguchi Y, Moriuchi T
机构信息
Division of Molecular Life Science (Cell Biology), Tokai University School of Medicine, Kanagawa, Japan.
出版信息
Nucleic Acids Symp Ser. 1992(27):153-4.
The triplex polymerase chain reaction (PCR) and high performance liquid chromatography (HPLC) techniques were used to examine the state of amplification of the c-myc gene in gastric carcinomas. Sequences from the c-myc gene and from the two control genes were coamplified by PCR. The coamplified PCR products were separated and quantified by HPLC and the copy numbers of the c-myc gene were calculated by comparing the peak areas generated by PCR products. Increased copy numbers of the c-myc gene were found in 2 of 5 patients.
采用三重聚合酶链反应（PCR）和高效液相色谱（HPLC）技术检测胃癌中c-myc基因的扩增状态。通过PCR对c-myc基因序列和两个对照基因序列进行共扩增。共扩增的PCR产物经HPLC分离和定量，并通过比较PCR产物产生的峰面积计算c-myc基因的拷贝数。5例患者中有2例发现c-myc基因拷贝数增加。
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